Skip to main content
. 2004 Feb 26;316(2):476–483. doi: 10.1016/j.bbrc.2004.02.074

Fig. 1.

Fig. 1

Yeast two-hybrid assay for N protein self-interaction. S. cerevisiae AH109 cells (1.5 ml of overnight culture) were transformed with 0.1 μg pGBK-N and pGAD-N plasmid DNA using the polyethylene glycol/lithium acetate method and plated on SD/LEU/−TRP and SD/−ADE−HIS/−LEU/−TRP drop-out plates and grown at 30 °C for 4 days. pGBK/T7 + pGAD/T7 and pGBK-M + pGAD-M were used as negative controls. Growth of the transformants on the SD/LEU/−TRP plate indicates that both pM- and pVP-related plasmids have been delivered into yeast cells, while the growth on SD/−ADE−HIS/−LEU/−TRP plate reveals activation of reporter genes, and thus interactions of proteins.