Table 2.

Sensitivity of NASBA-AIV and NASBA-H7 assays in detecting avian influenza H7N1 virus

Dilution	A/chicken/Italy/1081/99 (H7N1)a
	NASBA-AIV		NASBA-H7
	ECL signal	Result	ECL signal	Result
10−1	1,328,502	Positive	335,510	Positive
10−2	1,206,822	Positive	295,700	Positive
10−3	1,486,492	Positive	288,692	Positive
10−4	1,409,444	Positive	289,765	Positive
10−5	1,216,368	Positive	58,448	Positive
10−6	1,181,705	Positive	89,067	Positive
10−7	538	Negative	122	Negative
10−8	342	Negative	52	Negative
10−9	352	Negative	−115	Negative
10−10	288	Negative	69	Negative
IRS	54,715	NA	49,415	NA
Cut-off value (0.025 × IRS)	1367	NA	1235	NA

IRS, instrument reference solution.

NA, not applicable.

^a10^8.92 EID₅₀/ml.