Table 3.

Sensitivity of NASBA-AIV and NASBA-H5 assays in detecting avian influenza virus H5N3

Dilution	A/pekin duck/Singapore-Q/F119/3/97 (H5N3)a
	NASBA-AIV		NASBA-H5
	ECL signal	Result	ECL signal	Result
10−1	715,693	Positive	1,323,942	Positive
10−2	726,276	Positive	1,283,673	Positive
10−3	666,961	Positive	1,339,277	Positive
10−4	782,306	Positive	1,085,974	Positive
10−5	800,196	Positive	1,437,995	Positive
10−6	226	Negative	1,965,036	Positive
10−7	202	Negative	10,000,001b	Positive
10−8	277	Negative	65	Negative
10−9	180	Negative	162	Negative
10−10	232	Negative	269	Negative
IRS	45,712	NA	44,787	NA
Cut-off value (0.025 × IRS)	1142	NA	1120	NA

IRS, instrument reference solution

NA, not applicable.

^a10^8.92 EID₅₀/ml.

^bUpper limit of detection.