Table 5.
Specificity of NASBA-H7 assay in detecting distinct lineages of influenza virus and clinically relevant pathogens
| Sample |
HA subtype |
NASBA |
|
| ECL signal |
Result |
||
| A/ostrich/SA/1609/91 | H7N1 | 488,880 | Positive |
| A/psittacine/Italy/2/91 | H7N2 | 606,191 | Positive |
| A/turkey/N. Ireland/VF-1545 C5/98 | H7N7 | 270,511 | Positive |
| A/turkey/England/262/79 | H7N3 | 99,776 | Positive |
| A/ostrich/RSA/W-4/96 | H7N7 | 370,427 | Positive |
| A/blackbird/Singapore/F92/9/94 | H7N1 | 256,342 | Positive |
| A/bird/Pakistan/447/95 | H7N3 | 372,163 | Positive |
| A/equine/Prague/1/56a | H7N7 | 188 | Negative |
| A/chicken/Bendigo/Victoria/85 | H7N7 | 86,424 | Positive |
| A/chicken/Pakistan/5/99 | H9 | 140 | Negative |
| A/chicken/Pakistan/3/99 | H9 | 129 | Negative |
| A/chicken/Korea/25232-006/96 | H9 | 161 | Negative |
| A/Bayern/7/95 | H1 | 121 | Negative |
| A/Singapore/1/57 | H2 | 118 | Negative |
| A/chicken/RSA/01 | H6 | 193 | Negative |
| A/duck/Singapore-Q/F119-3/97 (H5N3) | H5 | 121 | Negative |
| Herpes (11T) 1994 | NA | −81 | Negative |
| Canary Pox 1997 | NA | 114 | Negative |
| Parvovirus (GPV) | NA | 66 | Negative |
| Picornavirus (IAE) 1995 | NA | 81 | Negative |
| Pneumonia (APV F83) subtype A | NA | 124 | Negative |
| Reovirus (S1133) | NA | 89 | Negative |
| Rotavirus (353/87) | NA | 155 | Negative |
| Coronavirus (793/B) 1991 | NA | 100 | Negative |
| Adenovirus (CELO) | NA | 110 | Negative |
| Instrument Reference Solution | NA | 51,764 | NA |
| Cut-off limit (0.025 × IRS) | NA | 1294 | NA |
NA, not applicable.
a
This reference strain was not detected by the NASBA-H7 assay.