Figure 3.

Impact of FRC-Specific LTβR Signaling on Peripheral LN Structure

(A) Inguinal LNs from 4-week-old Ccl19-cre × Ltbr^fl/fl and Ltbr^+/+ controls were analyzed by OPT for the presence of the HEV network (MECA-79⁺) and B cell follicles (B220⁺); scale bar represents 500 μm. Quantitative analysis based on OPT data for (B) LN volume, (C) B cell follicle volume, (D) HEV network length, and (E) HEV branching points (mean ± SEM, n = 6 mice pooled from two independent experiments).

(F) Confocal microscopic analysis of inguinal LNs from 4-week-old Ccl19-cre × Ltbr^fl/fl and Ltbr^+/+ controls by using antibodies against T cells (CD4) and B cells (B220); scale bar represents 200 μm.

(G) Flow cytometry-based quantification of LN cellularity. Values indicate relative cell numbers in Ccl19-cre × Ltbr^fl/fl mice compared to Ltbr^+/+ mice (CD4, CD4⁺ T cells; CD8, CD8⁺ T cells; BC, B cells; DC, dendritic cells; MP, macrophages; mean ± SEM, n = 6 mice from two independent experiments).