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. 2003 Oct 24;311(3):759–766. doi: 10.1016/j.bbrc.2003.10.066

Fig. 3.

Fig. 3

Mobility-shift analysis of the complete 3′UTR and the 3′UTR (A) of NV and S10 extract from HeLa cells. (A) [α-32P]UTP labeled 3′UTR (lanes 1 and 2) or 3′UTR(A) RNAs were incubated in the absence (lane 1) or presence of 20 μg of S10 extract from HeLa cells (lane 2 and 3) followed by RNAse treatment. (B) [α-32P]ATP labeled 3′UTR (lane 3) or 3′UTR(A) RNAs (lanes 1 and 2) were incubated in the absence (lane 1) or presence of 10 μg of S10 extract from HeLa cells (lanes 2 and 3) followed by RNAse treatment. Complex formation was assayed by electrophoresis on native polyacrylamide gels and detected by autoradiography. Mobility of complexes is indicated on both sides of the figure.