Table 1.
Summary of Data Collection, Structural Refinement, and Analysis
| GCN4-pVe/GCN4-pVg | GCN4-pAe/GCN4-pAg | |
|---|---|---|
| Resolution (Å) | 45.2−1.70 (1.76−1.70) | 56.2−1.70 (1.74−1.70) |
| Space group | P1 | P4212 |
| Unit cell parameters | a = 48.85 Å, b = 49.11 Å, c = 51.92 Å, α = 115.8°, β = 94.2°, γ = 109.6° | a = b = 79.47 Å, c = 54.95 Å |
| Number of unique reflections | 41,918 (4137) | 19,756 (1281) |
| Multiplicity | 2.0 (2.0) | 9.2 (9.0) |
| Rmerge (%) | 4.2 (41.3) | 5.5 (55.6) |
| I/σI | 12.7 (2.1) | 15.2 (4.9) |
| Completeness (%) | 97.0 (95.8) | 99.7 (99.9) |
| Number of molecules in the asymmetric unit | 12 | 5 |
| Solvent content (%) | 44.7 | 46.0 |
| Number of atoms in refinement | 3163 | 1256 |
| Number of solvent molecules | 405 | 130 |
| Rcryst, Rfree (%) | 17.1, 22.1 | 20.3, 23.0 |
| B protein (Å2) | 30.6 | 37.3 |
| Rmsd from ideal geometry | ||
| Bond lengths (Å) | 0.02 | 0.017 |
| Bond angles (°) | 1.5 | 1.5 |
| B values (Å2) | 3.9 | 3.7 |
Values for the highest-resolution shell are given in parentheses.