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Expression of SARS-CoV nucleocapsid protein. (A) SARS-CoV nucleocapsid protein was expressed in E. coli JM109 and purified using Ni–NTA–agarose. (B) 293T cells were mock-transfected with pcDNA3.1 empty vector (lane 1) or transfected with cDNA3.1-nucleocapsid construct (lane 2). Thirty hours after transfection, cell extracts were prepared and Western blot analysis was performed using affinity-purified anti-nucleocapsid polyclonal antibody.
