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The subcellular localization of nucleocapsid protein. (A) BIOCHIPS coated with SARS-CoV infected Vero E6 cells were immunostained with affinity-purified anti-nucleocapsid antibodies. The DAPI staining represents the position of DNA (A′). Human 293T (B) and green monkey Vero cells (C) were transfected with pcDNA3.1-nucleocapsid expression construct. Thirty hours post-transfection, cells were fixed in cold methanol and immunostained.
