Scheme 1.

Substrate scope of Mb(H64V,V68A)[Fe(DADP)] (2) and Mb(H64V,V68A,H93NMH)[Fe(DADP)] (3).^a

^a Reaction conditions: 10 mM alkene, 20 mM EDA, 10 μM Mb variant 2 or 3 in KPi buffer (50 mM, pH 7), r.t., 16 hrs, anaerobic conditions. Yield and diastereomeric excess were determined by chiral GC-FID analysis. Enantiomeric excess was determined by chiral GC-FID or SFC analysis. ^b 50 mM alkene, 20 mM EDA, 20 μM catalyst 3.