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. 2016 Apr 26;494:225–235. doi: 10.1016/j.virol.2016.04.018

Fig. 7.

Fig. 7.

Peptide substrate cleavage assays. A. Cleavage of coronavirus 3CLpro N-terminal substrate. Two fluorogenic peptide substrates (Dabcyl-KTSAVLQ↓SGFRKME-Edans and Dabcyl-YNSTLQ↓AGLRKME-Edans contain the N-terminal auto-cleavage site of PEDV and SARS-CoV 3CLpro, respectively) were introduced in the FRET assays. (□: SARS-CoV 3CLpro-derived substrate cleaved by PEDV 3CLpro; ○: PEDV 3CLpro-derived substrate cleaved by PEDV 3CLpro; Δ: SARS-CoV 3CLpro-derived substrate cleaved by SARS-CoV 3CLpro; ∇: PEDV 3CLpro-derived substrate cleaved by SARS-CoV 3CLpro). B. Cleavage of NEMO-derived substrate. Similar results were obtained when the concentration of the proteases increased by 10-folds (data not shown). Four proteases used in each assay were labeled in the figure. Motif-mutant and M25T represent two PEDV 3CLpro variants described in the text. Same concentration of the NEMO-derived substrate was used for the four proteases.