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. 2016 Jan 14;164(1):258–268. doi: 10.1016/j.cell.2015.12.044

Figure 2.

Figure 2

Binding Affinity of GPcl Bound to NPC1-C

(A) BIAcore diagram of refolded E. coli-expressed NPC1-C protein bound to the GP-ectoΔmucin protein. The refolded NPC1-C shows no binding to the GP-ectoΔmucin.

(B and C) BIAcore diagram and saturation curve of refolded NPC1-C protein bound to the GPcl protein. The refolded NPC1-C binds to the GPcl with a low affinity and a fast kinetics.

(D) BIAcore diagram of glycosylated 293T-expressed NPC1-C protein bound to the GP-ectoΔmucin protein. The glycosylated NPC1-C also shows no binding to the GP-ectoΔmucin.

(E and F) BIAcore diagram and saturation curve of glycosylated NPC1-C protein bound to the GPcl protein. The glycosylated NPC1-C binds to the GPcl with a similar low affinity and a fast kinetics. Response units were plotted against protein concentrations. The KD values were calculated by the BIAcore 3000 analysis software (BIAevaluation Version 4.1).

See also Figures S4 and S5.