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. 2016 Jan 14;164(1):258–268. doi: 10.1016/j.cell.2015.12.044

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Copyright © 2016 Elsevier Inc. All rights reserved.

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Figure S1 — Characterization of Refolded NPC1-C Protein and Analysis of Potential N-Link Glycosylation Sites, Related to Figure 1

(A) The refolded NPC1-C protein was eluted as a single sharp peak in the gel filtration on Superdex 200^® column (GE Healthcare). The NPC1-C protein shows perfect purity, which is ran as a single band in the SDS-PAGE without any extra bands. Irrelevant lanes were removed during preparation of the figure.

(B) The NPC1-C has seven potential N-link glycosylation sites, six of which locate in the loops except one (N452) at the tip of α4 helix. The sites are shown in red spheres in the NPC1-C structure.