Table 2.
Occurrence frequencies (%) of bacterial pathogen genes detected in surface waters by P/A PCR in the South Fork River Basin. Three pathogens genes (vanB, STII, STh) were not detected in any water samples at any of South Fork River Basin sites. The pathogen gene vanB was detected in both water and sediment samples at the WC site during August 2011. The STII gene was detected once in sediment at the TC313 site during November 2011 after fall manure application. Dashes indicate non-detect.
| Sampling date |
C. jejuni or coli |
Enterococcus |
E. coli |
Salmonella |
S. aureus |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 16S | esp human | vanA | ddl faecalis | ddl faecium | hirae bov | 16S | LTIIa bov | rfb O157 | stx1 | stx2 | stx2e | eaeA | invA | spvC | femA | mecA | |
| 8/09/2011 (pre manure) n = 5 | – | – | – | 20 | 80 | – | 100 | 20 | 20 | 40 | 20 | – | 100 | 20 | 20 | 40 | – |
| 11/03/2011 (post manure) n = 5 | 40 | 20 | – | 60 | 100 | 60 | 100 | 20 | – | – | – | 20 | 80 | 20 | – | – | 20 |
| 3/07/2012 (pre manure) n = 5 | – | – | 20 | 100 | 100 | – | 100 | – | 20 | 80 | – | – | 80 | 40 | – | – | – |
| 4/15/2012 (post manure) n = 5 | 40 | – | 20 | 80 | 80 | 80 | 100 | – | 40 | 100 | 60 | – | 100 | – | – | 60 | 60 |
| All dates (n = 20) | 20 | 5 | 10 | 65 | 90 | 35 | 100 | 10 | 20 | 55 | 20 | 5 | 90 | 20 | 5 | 25 | 20 |