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. 2017 Jan 17;184:42–53. doi: 10.1016/j.vetimm.2017.01.001

Fig. 3.

Fig. 3

Representative immunohistochemical reactions for detection of histiocytic cells in the spleen, lymph node, and thymus of a Bactrian camel (A-D: Iba-1; E-H: CD204). Avidin-biotin-peroxidase complex method with 3,3′-diaminobenzidine as chromogen. (A) Iba-1; spleen. Iba-1-specific immunohistochemistry shows a characteristic perivascular staining pattern (arrow), which is not observed for any other marker. Several immunopositive cells are also noted scattered throughout the splenic red pulp (RP), and perivascularly within the periarteriolar lymphoid sheath (PALS). Scale bar = 100 μm. (B) Iba-1; lymph node. Numerous cells stain positive for Iba-1 antigen within diffuse lymphoid tissue (DLT), which resembles the T cell compartment of camelids. Moderate numbers of scattered immunpositive cells are also noted within dense anodular lymphoid tissue (DALT). Scale bar = 100 μm. (C) Iba-1; thymus. A moderate number staining intensely positive for Iba-1 antigen are seen within both compartments of the thymus, cortex (C) and medulla (M). Scale bar = 100 μm. (D) Iba-1; lymph node. Higher magnification of the inset in (B). Morphologically, Iba-1 immunopositive cells are characterized by histiocyte-like morphology with abundant intensely and diffusely labeled cytoplasm and many cells show pronounced cytoplasmic projections. Scale bar = 20 μm. (E) CD204; spleen. Similar to Iba-1 but with absent perivascular staining pattern, several immunpositive cells are noted throughout the splenic RP with few immunoreactive cells in the PALS. Scale bar = 100 μm. (F) CD204; lymph node. CD204 immunopositive cells are mainly found within the DLT, while only few scattered cells are positive within the DALT. Scale bar = 100 μm. (G) CD204, thymus. In contrast to Iba-1 (C), a relatively lower number of CD204 immunpositive cells is noted within thymus medulla and cortex. Scale bar = 100 μm. (H) CD204; lymph node. Higher magnification of the inset in (F). CD204 positive cells are characterized by relatively high amounts of diffusely stained cytoplasm, peripheral nuclei, and histiocyte-like morphology. Scale bar = 20 μm.