Table 1.
Antigen, clonality, species, source, dilution, antigen retrieval and secondary antibodies used for IHC.
| antigen | clonality, clone, species | source | dilution | antigen retrieval | secondary Ab | result |
|---|---|---|---|---|---|---|
| CD3 | pc, rabbit | DakoCytomation | 1:500 | Citrat | GAR | + |
| CD79α | mc, HM57, mouse | Abcam | 1:5000 | Citrat | GAM | (+) |
| CD20 | pc, rabbit | Thermo Fisher scientific | 1:200 | Citrat | GAR | + |
| Pax-5 | mc, 24/Pax-5, mouse | BD Biosciences | 1:10 | # | GAM | – |
| Mum1 | mc, MUM1p, mouse | DakoCytomation | 1:10 | # | GAM | – |
| Foxp3 | mc, FJK-16s, rat | eBioscience | 1:10 | # | RAR | – |
| HLA-DR | mc, TAL.1B5, mouse | DakoCytomation | 1:100 | Citrat | GAM | + |
| Iba-1 | pc, rabbit | Wako | 1:500 | Citrat | GAR | + |
| CD68 | mc, KP1, mouse | DakoCytomation | 1:100 | Citrat | GAM | + |
|
Myeloid/histiocyte antigen |
mc, MAC387, mouse | DakoCytomation | 1:500 | Citrat | GAM | + |
| CD204 | mc, SRA-E5, mouse | Transgenic Inc. | 1:1000 | Citrat | GAM | + |
| CD205 | mc, CC98, mouse | AbD Serotec | 1:10 | # | GAM | – |
| CD208 | mc, 1010E1.01, rat | Dendritics | 1:200 | Citrat | RAR | + |
| Lysozyme | pc, rabbit | Dako | 1:10 | # | GAR | – |
CD, cluster of differentiation; Pax-5, paired box protein 5; MUM1, multiple myeloma oncogene 1; Foxp3, forkhead box P3, HLA, human leucocyte antigen; Iba-1, ionized calcium-binding adapter molecule 1; pc, polyclonal; mc, monoclonal; #, antigen retrieval was performed with boiling citrate buffer, pronase, and omission of pretreatment; GAR, goat anti-rabbit Immunoglobulin G (IgG); GAM, goat anti-mouse IgG; RAR, rabbit anti-rat IgG; +, positive; −, negative; (+) the antibody displayed a distinct immunoreaction on certain cells, but there was considerable background staining, false nuclear reactions, and the number of labeled cells was significantly lower than in the positive control of canine tissue.