Table 2.
Distribution of antigens in different compartments of spleen.
| Detected antigen | Follicles |
PALS |
Red Pulp Cords |
Red pulp Sinuses |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L | A | D | C | L | A | D | C | L | A | D | C | L | A | D | C | |
| CD3 | 1.00 | 1.00 | 0.66 | 1.00 | 3.00 | 3.00 | 2.60 | 3.00 | 1.60 | 1.80 | 1.60 | 1.60 | 1.00 | 1.00 | 1.20 | 1.40 |
| 0.00 | 0.00 | 0.47 | 0.00 | 0.00 | 0.00 | 0.49 | 0.00 | 0.49 | 0.40 | 0.49 | 0.49 | 0.00 | 0.00 | 0.75 | 0.49 | |
| CD20 | 3.00 | 3.00 | 3.00 | 3.20 | 1.60 | 1.80 | 1.20 | 1.00 | 1.80 | 1.80 | 1.00 | 1.60 | 2.00 | 1.40 | 0.80 | 1.00 |
| 0.63 | 0.00 | 0.71 | 0.75 | 0.49 | 0.40 | 0.40 | 0.00 | 0.40 | 0.40 | 0.63 | 0.49 | 0.63 | 0.80 | 0.40 | 0.00 | |
| CD79 | 1.80 | 2.00 | 1.25 | 0.25 | 1.20 | 0.80 | 0.40 | 0.50 | 1.60 | 2.00 | 1.00 | 1.00 | 1.00 | 0.20 | 0.00 | 0.00 |
| 0.40 | 0.00 | 0.83 | 0.43 | 0.40 | 0.75 | 0.49 | 0.50 | 0.49 | 0.00 | 0.63 | 0.00 | 0.89 | 0.40 | 0.00 | 0.00 | |
| Iba-1 | 1.20 | 1.00 | 1.00 | 1.00 | 3.00 | 1.80 | 2.20 | 2.60 | 2.00 | 2.00 | 1.80 | 2.00 | 1.00 | 1.20 | 0.60 | 1.20 |
| 0.40 | 0.00 | 0.00 | 0.00 | 0.00 | 0.40 | 0.75 | 0.80 | 0.00 | 0.00 | 0.40 | 0.00 | 0.63 | 0.40 | 0.49 | 0.75 | |
| CD204 | 0.60 | 1.00 | 1.00 | 0.80 | 1.20 | 1.00 | 1.40 | 0.80 | 1.60 | 1.80 | 1.60 | 1.80 | 0.60 | 1.00 | 0.80 | 0.60 |
| 0.49 | 0.00 | 0.00 | 0.40 | 0.40 | 0.63 | 0.49 | 0.40 | 0.49 | 0.40 | 0.49 | 0.40 | 0.49 | 0.89 | 0.40 | 0.49 | |
| m/h antigen | 0.80 | 0.67 | 1.00 | 1.00 | 1.00 | 1.00 | 1.40 | 1.00 | 2.00 | 1.80 | 1.80 | 1.20 | 1.20 | 0.60 | 0.80 | 0.80 |
| 0.40 | 0.47 | 0.00 | 0.00 | 0.63 | 0.00 | 0.49 | 0.00 | 0.00 | 0.40 | 0.40 | 0.40 | 0.40 | 0.80 | 0.40 | 0.75 | |
| HLA-DR | 2.20 | 1.67 | 2.00 | 1.80 | 2.00 | 2.60 | 1.80 | 2.80 | 1.80 | 1.20 | 1.40 | 1.80 | 0.20 | 0.00 | 0.40 | 0.00 |
| 0.75 | 0.94 | 1.00 | 0.98 | 0.00 | 0.49 | 0.40 | 0.40 | 0.40 | 0.40 | 0.49 | 0.40 | 0.40 | 0.00 | 0.49 | 0.00 | |
| CD208 | 0.00 | 0.00 | 0.00 | 0.00 | 0.20 | 0.00 | 0.00 | 0.00 | 0.40 | 0.20 | 0.40 | 0.40 | 0.40 | 0.20 | 0.00 | 0.00 |
| 0.00 | 0.00 | 0.00 | 0.00 | 0.40 | 0.00 | 0.00 | 0.00 | 0.49 | 0.40 | 0.49 | 0.49 | 0.49 | 0.40 | 0.00 | 0.00 | |
| CD68 | 0.00 | 0.00 | 0.00 | 0.20 | 0.60 | 0.00 | 0.00 | 0.60 | 0.80 | 0.80 | 0.60 | 0.80 | 0.20 | 0.00 | 0.00 | 0.00 |
| 0.00 | 0.00 | 0.00 | 0.40 | 0.49 | 0.00 | 0.00 | 0.49 | 0.40 | 0.40 | 0.49 | 0.40 | 0.40 | 0.00 | 0.00 | 0.00 | |
Averages (upper rows) and standard deviations (lower rows) of immunopositive cells were calculated from score values (0, no positive staining cells; 1, < 10%; 2, 10–50%; 3, 51–90%; 4, > 90% of immunolabeled cells) for different compartments of spleen. Thymus tissue was only available from a low number of animals and thus not included in the table. llamas; A, alpacas; D, dromedaries; C, Bactrian camels; PALS, periarteriolar lymphoid sheaths; CD, cluster of differentiation; HLA, human leucocyte antigen; Iba-1, ionized calcium-binding adapter molecule 1; m/h antigen, myeloid/histiocyte antigen.