Figure 1.

Overall Architecture of the Human NPC1

(A) The last step purification of full-length hNPC1 through size exclusion chromatography. The indicated fractions were applied to SDS-PAGE and visualized by Coomassie blue staining.

(B) The EM map of hNPC1 reconstructed at 4.4 Å resolution. The domain-colored maps were generated in Chimera (Pettersen et al., 2004). Unless otherwise indicated, the same domain color scheme is applied to all figures.

(C) The structural model of hNPC1. The structure on the left is rainbow colored with the amino and carboxyl termini colored blue and red, respectively. The invisible cytoplasmic segments connecting TM1 and α1-2 and between TM7 and α7-8 are indicated by dotted lines. The structure on the right is domain colored. The glycosyl groups are shown as black sticks. All structure figures were prepared with PyMol (DeLano, 2002).