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. 2016 May 26;165(6):1467–1478. doi: 10.1016/j.cell.2016.05.022

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© 2016 Elsevier Inc.

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Cryo-EM Architecture of the Complex between EBOV-GPcl and FL hNPC1

(A) Summary of the 3D classifications of the NPC1-GPcl complex. Please refer to Figure S7 for cryo-EM data processing.

(B) One EBOV-GPcl trimer binds to one NPC1 monomer. The 6.6 Å cryo-EM reconstruction of the hNPC1-GPcl complex obtained from the two classes indicated by the red box in (A).

(C) Structural model of the NPC1-GPcl complex. The trimeric crystal structure of EBOV-GPcl (PDB: 5F1B) and the EM structure of NPC1 were fitted to the cryo-EM map in Chimera.

(D) Deviation between the cryo-EM reconstruction and the docking model of GPcl in the complex. Despite the seeming contact between GPcl and NTD in the docking model (colored silver), the GPcl protomer (colored wheat) that contacts domain C slightly moves away from NTD in the cryo-EM reconstruction. Consequently, no direct contact between GPcl and NTD was seen in the EM map. It remains to be investigated whether glycosyl moieties may fill up the void between NTD and GPcl.