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. 2018 Oct 17;259:18–27. doi: 10.1016/j.virusres.2018.10.012

Fig. 1.

Fig. 1

Isolation and detection of the PEDV CH/HNPJ/2017 strain in Vero cells. The upper and lower panels show light and immunofluorescence images, respectively, of Vero cells infected with the PEDV CH/HNPJ/2017 strain. (A) Cytopathic effects (CPE) caused by the 1st passage of the PEDV CH/HNPJ/2017 strain at 24 h after inoculation (400×). Vero cells were seeded into T-25 flasks and infected at a multiplicity of infection (MOI) of 0.1; (B) CPE at 72 h after inoculation; (C) Control (uninfected) Vero cells; (D) Immunofluorescence detection results for the 50th passage of the PEDV CH/HNPJ/2017 strain in infected Vero cells at 6 h after inoculation (400×). PEDV antigens and nuclei were detected with mouse anti-PEDV N protein monoclonal antibodies (McAbs) and 4′, 6-diamidino-2-phenylindole (DAPI), respectively; (E) Immunofluorescence detection results at 12 h after inoculation (400×); (F) Immunofluorescence detection results at 24 h after inoculation (400×).