Table 1.
Cryo-EM Data Collection and Validation Statistics
| Data Collection and Processing | |
|---|---|
| EMDB | EMD-20536 |
| Microscope | FEI Titan Krios |
| Magnification | 22,500 |
| Pixel size (Å) | 1.075 |
| Voltage (kV) | 300 |
| Detector | Gatan K2 Summit |
| Exposure (e−/Å2) | 48 |
| Defocus range (μm) | −1.5 to −2.5 |
| Final particles | 196,720 |
| Symmetry | C1 |
| Resolution (Å) | 3.2 |
| Model Refinement and Validation | |
| PDB ID | 6PZK |
| Model Composition | |
| Non-hydrogen atoms | 13,153 |
| RMSD bonds (Å) | 0.012 |
| RMSD angles (°) | 1.94 |
| Ramachandran | |
| Favored (%) | 96.2 |
| Allowed (%) | 3.8 |
| Outliers (%) | 0 |
| Rotamer outliers (%) | 1.95 |
| MolProbity score | 1.09 |
| EM Ringer score | 2.6 |