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. 2020 Mar 25;8:161. doi: 10.3389/fchem.2020.00161

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Copyright © 2020 Zhu, Wang, Xie, Qin, Lin and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative PCR analysis of the odontoblast differentiation markers ALP (A) and DSPP (B), and western blot analysis of osteo/odontogenic markers (C). The integrated density of bands were analyzed by ImageJ (D). The molecular weights of marked protein were listed followed: β-actin 45kD, OCN 50-55kD, DSPP 37-40kD, RUNX2 55-62kD. The asterisks indicate significant differences between groups (*p ≤ 0.05 one-way ANOVA and LSD comparison test) and the bars represent the standard deviation of three replicates.