Fig. 2.

Exogenous cholesterol replenishment restores virus infection. (A-C) Vero, H1299 cells, and Huh7 cells were pretreated with 5 mM MβCD for 30 min and supplemented with 1 mM cholesterol for 1 h before IBV infection. MβCD-treated cells without cholesterol replenishment were included in a parallel experiment as control. The entry of IBV was measured by quantification of viral gRNA at 2 h.p.i.; the expression of IBV N protein was measured at 8 h.p.i.; the release of virus particles in the medium was measured with TCID₅₀ assay at 12 h.p.i. The experiment was performed in triplicate, and average values with stand errors were presented in bar graph panel. The intensity of IBV N band was determined with Image J, normalized to β-actin, and shown as fold change of MβCD : H₂O.