Table 2.

Measured and expected C_T values for SIV gag RNA in duplex RT-qPCR.

Samplea	CDV N copies	SIV gag copies	SIV gag measured CTc	SIV gag expected CTb,c
1:100	107	107	15.53	16.00
1:10−1	107	106	18.63	19.79
1:10−2	107	105	22.24	23.39
1:10−3	107	104	26.74	26.63
1:10−4	107	103	30.90	30.43
1:100	104	104	25.33	26.63
1:10−1	104	103	29.10	30.43
1:10−2	104	102	33.78	33.11
1:10−3	104	101	37.69	36.48

^aRatios of CDV N to SIV gag.

^bExpected C_T values were derived from standard curves based on the duplex RT-qPCR assay in Fig. 2C.

^cAll C_T values are means of duplicates.