Table 3.
Evaluation of the CDV-specific mRNA RT-primer in the 2-step singleplex and duplex RT-qPCR assays.
| Samplesa | CTb for singleplex assayc |
CTb for duplex assayd |
||
|---|---|---|---|---|
| CDV N VIC | SIV gag FAM | CDV N VIC | SIV gag FAM | |
| Infected Vero cells 1 | 13.6 | 12.8 | 13.7 | 12.0 |
| Infected Vero cells 2 | 13.0 | 12.4 | 12.4 | 12.0 |
| Uninfected cells | – | – | – | – |
| 5 × 108 copies gRNA | – | – | – | – |
a
Vero cells infected with rCDV-SIVgag1.
b
CT, threshold cycle number at which fluorescence was detected above the background. Positive CT values are ≤40 while negative CT values are >40 and are shown by a dash line.
c
Separate singleplex reactions for CDV N and SIV-gag were carried out in different wells with specific primer-probe sets.
d
Duplex reactions for CDV N and SIV-gag were done in the same wells containing primer-probe sets specific for both genes.