Skip to main content
. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Am Mosq Control Assoc. 2014 Mar;30(1):21–30. doi: 10.2987/13-6386.1

Fig. 4.

Fig. 4.

Real-time reverse transcriptase polymerase chain reaction (RT-PCR) results from the processing variables evaluation of mosquito pools containing 1, 5, 25, and 50 individuals homogenized in BA-1 cell culture medium by Vortex (1 min) or Mixer Mill (4 min at 25 cycles/sec). CT values ≤ 37 are considered positive (represented by the dashed line in each panel). Samples that were processed in BA-1 are represented by black diamonds (◆); samples processed in RAMP buffer are shown as gray diamonds (◆).