Skip to main content
. 2020 Apr 1;100(8):1030–1041. doi: 10.1038/s41374-020-0420-9

Fig. 1. Positive and negative controls for the PLAs.

Fig. 1

Signal positive (a–c) and signal negative (d) controls of the proximity ligation assays (PLA) were established using HUVECs. Mouse anti-talin antibodies were titrated at 1:50 (a), 1:500 (b), and 1:2000 (c) during PLA assay. There is no positive signal visualized during PLA using mouse anti-Rab5 and rabbit anti-vWF in HUVECs (d) and mouse liver tissue (h). Normal mouse and rabbit IgGs were used as reagent negative controls during PLA assay in HUVECs (e). IF to von Willebrand factor (arrowheads in f) and Rab5 (arrowheads in g) were processed in HUVECs. Nuclei were counter-stained with DAPI (blue). Scale bars indicate 20 µm.