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. 2020 Apr 1;100(8):1030–1041. doi: 10.1038/s41374-020-0420-9

Fig. 2. Quality controls for PLA in mouse tissue using primary antibodies raised in mouse.

Fig. 2

During PLA assay on mouse liver tissue samples from SFGR infection mouse on day 19, there is no specific signals if no primary antibody employed (only normal mouse and rabbit IgGs) (a), using rabbit anti-vinculin antibodies and normal mouse IgG employed (b), using rabbit anti-vinculin antibodies and mouse anti-talin, but no oligonucleotide probes (e). There is specific signal detected when rabbit anti-vinculin and mouse anti-talin antibodies were employed during PLA on mouse liver tissue (c, d). Nuclei were counter-stained with DAPI (blue). Scale bars indicate 20 µm.