Fig. 4.

DP2392-E10 inhibits NP/CRM1 binding by directly targeting CRM1. (A) FLAG– or NP-FLAG–immobilized agarose beads were incubated with purified CRM1-HA protein in the presence of DMSO or 3, 10, 30, or 100 µM of DP2392-E10 overnight, pulled down, washed, and subjected to western blot analysis with anti-HA and anti-FLAG antibodies. Intensity of the pulled-down CRM1 and NP were quantified using the ImageJ software. Data are representative of two independent experiments. (B) Photo-crosslinked Sepharose beads without DP2392-E10 (control) or with DP2392-E10 were co-incubated with purified NP-FLAG or FLAG-CRM1 proteins, pulled down, washed, and subjected to western blot analysis with anti-FLAG antibody. Data are representative of two independent experiments.