Fig. 10.

Inhibition of ERK1/2 activation does not affect PEDV-induced apoptosis. Vero cells were treated with DMSO, U0126 (20 μM), or PD98059 (100 μM) for 1 h prior to infection and then mock-infected or infected with PEDV (MOI of 1) in the presence of DMSO or each inhibitor. (A) Cells were harvested at the indicated time points, dually labeled with Annexin V and PI, and subjected to FACS analysis. The graph represents the percentage of each quadrant, and the non-significant percentages of Annexin V-negative and PI-positive cells were excluded. (B) The treated and infected cells were also labeled with MitoTracker Red CMSRos (Red), fixed, and incubated with an anti-AIF antibody (green). AIF nuclear translocation is represented by the merged AIF and DAPI signals (turquoise), and residual mitochondrial colocalization is indicated by the merged AIF and mitochondrial marker (yellow). The inset images are enlarged versions of parts of the picture.