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SDS–PAGE analysis of recombinant rPcL-lectin protein and the specificity of its anti-serum. pGEX-Llec-DE3 represents control group, rPcL-lectin protein was not expressed without IPTG induction. pGEX-Llec-DE3+IPTG represents experiment group, rPcL-lectin protein was expressed in E. coli with IPTG induction. GST-L-lec represents purified rPcL-lectin protein from E. coli, L-lec represents PcL-lectin, antiserum could recognize endogenous L-lectin from the crayfish.
