Fig. 2.

Tissues distribution of PcL-lectin. (A) The expressions of PcL-lectin mRNA in the six tissues of crayfish. The extracted total mRNAs from hemocytes, heart, hepatopancreas, gills, stomach and intestine were subjected to qRT-PCR assay. The 18S rRNA was used as reference. (B) The expression of PcL-lectin protein in the six tissues of crayfish. The extracted total proteins from the six tissues were subjected to Western blot assay. β-actin was used as internal control. The experiments were repeated three times. Different letters represented significant changes between groups, p < 0.05.