Fig. 5.

Recombinant PcL-lectin protein and WSSV replication. Crayfish was divided into four groups. Group 1 was control, Group 2 was injected with PBS and WSSV, Group 3 was injected with GST-tag and WSSV, Group 4 was injected with GST-PcL-lectin and WSSV. (A) Genome DNA was extracted from Gills, and qPCR was used to detect the amount of WSSV (vp28 mRNA as the marker for WSSV replication). (B)Total protein was extracted from gills of crayfish, and western blot was used to detect the amount of WSSV (VP28 as a marker for WSSV). Each experiment was repeated three times. Different letters represented significant changes between groups, p < 0.05.