Fig. 6.

PcL-lectin knockdown inhibited WSSV in crayfish. The crayfish were subjected to RNA interference with dsGFP as the control. (A and B) Effect of PcL-lectin RNAi on gills determined using qPCR and Western blot analysis. (C)The crayfish were infected with WSSV after PcL-lectin knockdown, and the amount of WSSV was detected via western blot using VP28 as a marker. (D)The rescue experiment was also performed through the injection of rPcL-lectin into the PcL-lectin-silenced and GFP-silenced crayfish; GST-tag was injected into the PcL-lectin-silenced and GFP-silenced crayfish as controls. Different letters represented significant changes between groups, p < 0.05.