Fig. 4.

NNV infection enhances Drp1 phosphorylation and its mitochondrial translocation. A. Immunofluorescence analysis at 48 hpi showing the induction of drp1 Ser616 phosphorylation in NNV-infected SISS cells. Mitochondria stained with Mitotracker green, nucleus with DAPI and anti Drp 1 probed with TRITC labeled secondary antibody. B. SISS cells infected with NNV and mitochondria was extracted. Association of phosporylated Drp 1 in the mitochondrial fraction determined by western blot. VDAC-1 used as mitochondrial marker. C. Time dependent real time mRNA expression analysis of mitochondrial fission factors in NNV infected cells. Expression was normalized with EF1α as the internal control. Data are expressed as means with SEM of three independent experiments (p < 0.05), * indicates significance difference.