Fig. 1.

VP56 is stimulated by virus infection. . (A) qPCR detection of the transcriptional levels of s7 on stimulation. GCO cells were seeded on 6-well plates overnight and infected with GCRV (100 μl of the filtered virus-containing supernatant of frozen and thawed GCO cells, which was diluted 100 times with PBS) At the time points 0, 1, 2, 3, 5 and 7 day, total RNA was extracted for further qPCR assays.(B and C) VP56 inhibits poly I:C-induced IFN expression. GCO cells seeded into 6-well plates overnight were transfected with 2 μg Myc-VP56 or the empty vector and transfected with poly I:C (2 μg/ml) at 24 h post-transfection. At 24 h post transfection, total RNAs were extracted to examine the transcriptional levels of cellular ifn and isg15 by qPCR. The relative transcription levels were normalized to the transcription level of the β-actin gene and are represented as fold induction relative to the transcription level in control cells, which was set to 1. Data were expressed as mean ± SEM, n = 3. Asterisks indicate significant differences from control (*, p < 0.05). All experiments were repeated for at least three times with similar result.