Fig. 2.

VP56 inhibits MAVS-mediated IFN1 activation. (A and B) EPC cells were seeded on 24-well plates overnight and co-transfected with MAVS, IRF7, and pcDNA3.1-VP56 or pcDNA3.1 (+) plus IFN1pro (A) or ISRE-Luc (B) at the ratio of 1:1:1 (0.5 μg for each). pRL-TK was used as a control. At 24 h post transfection, cells were collected for detection of luciferase activities. The promoter activity is presented as relative light units normalized to Renilla luciferase activity. Data were expressed as mean ± SEM, n = 3. Asterisks indicate significant differences from control (*, p < 0.05). All experiments were repeated for at least three times with similar result.