Fig. 6.

VP56 dampens the cellular IFN response and facilitates SVCV proliferation. (A and B) EPC cells seeded into 6-well plates overnight were infected with SVCV (MOI = 1). After 24 h, total RNAs were extracted to examine the transcriptional levels of cellular epcifn and epcvig1 by qPCR. The relative transcription levels were normalized to the transcription level of the β-actin gene and are represented as fold induction relative to the transcription level in control cells, which was set to 1. Data were expressed as mean ± SEM, n = 3. Asterisks indicate significant differences from control (*, p < 0.05). (C and D) EPC cells seeded in 24-well plates overnight were transfected with 0.5 μg pcDNA3.1 (+)-VP56 or pcDNA3.1 (+) vector. At 24 h post-transfection, cells were infected with SVCV (MOI = 0.001) for 48 h. (C) Then, cells were fixed with 4% PFA and stained with 1% crystal violet. (D) Culture supernatants from the cells infected with SVCV were collected, and the viral titer was measured by standard TCID₅₀ method. All experiments were repeated for at least three times with similar results. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)