Skip to main content
. 2015 Nov 27;488:129–136. doi: 10.1016/j.virol.2015.11.008

Fig. 1.

Fig. 1

Affinity of Tomato chlorosis virus p22 to 562 bp, 100 bp dsRNAs, and 21 bp sRNAs. Representative EMSA assays with (a) 562 bp DIG-labeled dsRNA, (b) 100 bp DIG-labeled dsRNA, and (c) 21 nt dsRNA DIG-labeled at the 3’-end of the antisense overhang. The protein S-adenosylhomocysteine hydrolase (SAHH) was used as a negative control. The concentrations of the p22 and SAHH proteins are indicated above each lane. The RNA/proteins were transferred to nylon membranes, and dsRNAs were detected using an anti-digoxigenin antibody and a chemiluminescent substrate.