Skip to main content
. 2015 Nov 27;488:129–136. doi: 10.1016/j.virol.2015.11.008

Fig. 2.

Fig. 2

Affinity of Tomato chlorosis virus p22 to 562 nt or 100 nt ssRNAs and to 562 nt ssDNA and dsDNA. Representative EMSA assays with (a) 562 nt DIG-labeled ssRNA, (b) 100 nt DIG-labeled ssRNA, (c) 562 nt DIG-labeled ssDNA, and (d) 562 nt DIG-labeled dsDNA. The protein S-adenosylhomocysteine hydrolase (SAHH) was used as a negative control. The concentrations of the p22 and SAHH proteins are indicated above each lane. The nucleic acids/proteins were transferred to nylon membranes, and dsRNAs were detected using an anti-digoxigenin antibody and a chemiluminescent substrate.