Table 2.
Adventitious mutations found in the S genes of recombinant virusesa
| Virus | Mutationsb (nt) | Substitutions (aa) |
|---|---|---|
| SA59 A | A254G | N85S |
| SA59 Bc | − | − |
| S33R A | − | − |
| S33G A | A2350T | T784S |
| S33G B | A2511G | S837S |
| S33G C | − | − |
| T62S A | − | − |
| T62A A | − | − |
| L65H A | T1626C | − |
| C3074A | A1025D | |
| L65H B | G857T | S286I |
| L65H C | − | − |
| L65A A | G2500T | D834Y |
| L65A B | C3116T | A1039V |
| L65A C | − | − |
| L79M/T82M A | − | − |
| L79M/T82M B | − | − |
| L79M/T82M C | − | − |
| L79A/T82A A | C1010A | A337D |
| L79A/T82A B | − | − |
| Y162F A | C939A | − |
| K183R A | T2874A | − |
| K183G A | − | − |
| K183G B | − | − |
| K183G C | − | − |
(−) indicates no adventitious mutations.
a
S gene sequences are numbered according to GenBank accession no. AY497328.
b
S genes of all recombinant viruses contained engineered mutations introduced using targeted RNA recombination.
c
Recombinant viruses indicated in bold were characterized further in infection, neutralization and antibody blockade experiments.