Table 3.
Oligonucleotides used for site directed mutagenesis and sequencing
| Primer | Sequence (5′–3′)a |
|---|---|
| Forward | |
| K183G+ | GCACACGGATGTAggACCCCCAATTTGTG |
| K183R+ | GCACACGGATGTAAggCCCCCAATTTGTG |
| L65A+ | GCCACATTATTGgcTACTGGTTACTACCCG |
| L65H+ | GCCACATTATTGCaaACTGGTTACTACCCG |
| L79A/T82A+ | GTTTAGAAACgcCGCTCTTgCGGGAACTAACTC |
| L79M/T82M+ | GTTTAGAAACaTgGCTCTTAaGGGAACTAACTC |
| S33G+ | GGTGCTAATGTTgGTGCTCCAAGCATTAGC |
| S33R+ | GGTGCTAATGTTcGTGCTCCAAGCATTAGC |
| S(NotI)+ | GCTGTTATAGcGGCCGcGTTTCTGCTGC |
| S(XbaI)+ | GCTTAGGTGTTcTAGAAGATAATTGTGG |
| T62A+ | CGAGTTTATTTAAATGCCgCtTTATTGCTTACTGG |
| T62S+ | CGAGTTTATTTAAATGCCtCtTTATTGCTTACTGG |
| Y162A+ | CCATTTGTCAGTTACCTgcCACTGATTGTAAGC |
| Y162F+ | CCATTTGTCAGTTACCTTcaACTGATTGTAAGC |
| Y162H+ | CCATTTGTCAGTTACCTcACACTGATTGTAAGC |
| Y162Q+ | CCATTTGTCAGTTACCTcAaACTGATTGTAAGC |
| Reverse | |
| 572 | TCGCTTTAACACACAAATTGG |
| BZ25 | CCGTCTAGATATGTGGATCTTTTCAACGTC |
a
Nucleotides that differ from the S gene sequence of MHV-A59 (GenBank accession no. AY497328) are shown in lower case. Nucleotides that encode amino acid substitutions are underlined.