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. 2005 Jul 25;340(2):174–182. doi: 10.1016/j.virol.2005.06.016

Fig. 1.

Fig. 1

VSV recombinant expressing the SARS-CoV S protein. (A) The diagram illustrates the genome of the VSV-S recombinant expressing the S gene and shows the terminal coding and flanking VSV transcription start and stop signals. (B) BHK-21 cells were infected with wt VSV (lanes 1 and 2) or VSV-S (lanes 3 and 4), labeled with [35S]-methionine, and lysates were analyzed by SDS–PAGE without (lanes 1 and 3) or with immunoprecipitation with anti-S antibody (lanes 2 and 4). (C) Immunoprecipitated S was left undigested (lane 1), digested with PNGase F (lane 2), or Endo H (lane 3).