Table 2.
Pig no. | 1 | 2 | 3 | 4, 5 and 6 | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Oral inoculum, log10 GE/ml |
2 ml, 8.9 (9.2 log10 GE/pig) |
2 ml, 8.9 (9.2 log10 GE/pig) |
2 ml, 8.9 (9.2 log10 GE/pig) |
2 ml, mock |
||||||||||||||||||||
Onset of vomitinga |
None (>PIH 16) |
None (>PIH 16) |
Around PIH 16 |
None |
||||||||||||||||||||
Fecal consistency score at PIH 16b |
0 |
0 |
1 |
0 |
||||||||||||||||||||
PEDV RNA in serum, log10 GE/ml, at PIH 16c |
8.4 |
8.1 |
8.5 |
<3.8 |
||||||||||||||||||||
Distribution of PEDV-induced histologic lesions based on intestinal locations (as also determined by IHC data)d |
D |
PJ |
MJ |
DJ |
I |
C |
D |
PJ |
MJ |
DJ |
I |
C |
D |
PJ |
MJ |
DJ |
I |
C |
D |
PJ |
MJ |
DJ |
I |
C |
− |
− |
+ |
− |
+ |
− |
− |
− |
+ |
− |
+ |
− |
− |
+ |
+ |
− |
+ |
− |
− |
− |
− |
− |
− |
− |
|
Antigen detection by IHC in FFPE intestinal tissue sectionse |
+/− |
+ or ++ |
+++ |
+ or ++ |
++ or +++ |
− |
− |
+ |
+++ |
+ |
+ |
− |
+ |
+ or ++ |
+++ |
+ or ++ |
+ or ++ |
− |
− |
− |
− |
− |
− |
− |
D, duodenum; PJ, proximal jejunum; MJ, mid-jejunum; DJ, distal jejunum; I, ileum; C, colon; IHC, immunohistochemistry; FFPE, formalin-fixed, paraffin-embedded.
Pig 3 had only one frequency of vomiting until euthanasia. None of the pigs 1–6 had other clinical signs until euthanasia.
Fecal consistency was scored as follows: 0 = solid; 1 = pasty; 2 = semi-liquid; 3 = liquid, with scores of 2 or more considered diarrheic.
Detected by real-time reverse transcription PCR with a detection limit of 4.8 log10 GE/ml for fecal samples and 3.8 log10 GE/ml for serum samples.
Detected by histopathology as evaluated along with IHC data. +, swelling and coagulative necrosis of villous epithelial cells, subepithelial edema, exfoliation of enterocytes, and/or villous atrophy; and -, no such lesions or lipid deposit in enterocytes.
Detected by IHC in multiple tissue sections, resulting in a range of IHC score. +/− (few), <1% of villous epithelial cells showed staining; + (low), 1%–29% of villous epithelial cells showed staining; ++ (moderate), 30%–59% of villous epithelial cells showed staining; +++ (high), 60%–100% villous epithelial cells showed staining; and -, no cells showed staining.