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Proteolytic processing in furin-deficient FD11 and wild-type CHO cells. Wild-type S glycoprotein and the furin-site-containing mutants HTVR, HTR and SSLR were metabolically labeled in furin-deficient cells and parental CHO cells (Gordon et al., 1995). The S glycoprotein in cell lysates was affinity isolated (top panels), and the S1 subunit in the cell culture medium (bottom panels) was immunoprecipitated using MAb F26G18 (Berry et al., 2004).
