Table 2.
Primer nucleotide sequences used for the amplification of FCV, FHV-1, FPV and FCoV.
| Virus | Sequence |
|---|---|
| FCVa | Primer forward: GNA AAG CWC AAC AAA TTG AATT Primer reverse: CHTGTACCCTYTGCTCAAG |
| FHV-1b | Primer forward: ACGTGGTGAATTATCAGCTGAAG Primer reverse: AAGGTATGGTGCGGCAAATC Probe: TGCTGCCTATATCACCGCCCACTATCAA |
| FPVb | Primer forward: CAGGAAGATATCCAGAAGGA Primer reverse: GGTGCTAGTTGATATGTAATAAACA |
| FCoVb | Primer forward: TGGTCATCGCGCTGTCTACT Primer reverse: AGGGTTGCTTGTACCTCCTATTACA Probe:TTGTACAGAATGGTAAGCAC |
b
In house designed.