Table 3.
PCR and real time PCR amplification conditions.
| Virus | Reverse transcription | Initial denaturation | Denaturation | Annealing | Extension | Last Extension |
|---|---|---|---|---|---|---|
| FCV | 42 °C/30 mn | 95 °C/5 mn | 95 °C/30 s 35 Cycles |
52 °C/30 s 35 Cycles |
68 °C/30 s 35 Cycles |
68 °C/10 mn |
| FHV-1 | 95 °C/10 mn | 95 °C/15 s | 60 °C/1 mn 45 Cycles |
65 °C–95 °C (0.1 °C/s ramp rate) |
||
| FPV | 95 °C/10 mn | 94 °C/30 s 40 Cycles |
50 °C/1 mn 40 Cycles |
68 °C/1 mn 40 Cycles |
68 °C/10 mn | |
| FCoV | 48 °C/15 mn | 95 °C/10 mn | 95 °C/15 s | 60 °C/1 mn 45 Cycles |
65 °C–95 °C (0.1 °C/s ramp rate) |
|