Fig. 4.

Wild type DC-SIGNR and DC-SIGNR alleles with five and six repeat units augment viral infectivity with comparable efficiency. (A) Enhancement of filovirus glycoprotein and SARS-CoV-S dependent infection. The indicated lectins were transiently expressed on 293T cells, the cells infected with lentiviral reporter viruses bearing the indicated glycoproteins and luciferase activities in cellular lysates determined. The results of a representative experiment performed in quadruplicates are shown, error bars indicate standard deviation (SD). Similar results were obtained in two independent experiments. (B) Augmentation of HIV infection. The indicated lectins were transiently expressed on 293T cells, the cells pulsed with replication competent HIV-1 reporter virus, washed, cocultivated with CEMx174 5.25 target cells and luciferase activities in the cocultures determined. The results of a representative experiment carried out in quadruplicates are shown and were confirmed in two independent experiments. Error bars indicate SD.