Fig. 5.

Replacement of the mDC-SIGN CRD with the CRD of hDC-SIGN confers binding to soluble EBOV-GP. (A) Expression of chimeric lectins. The expression of the indicated lectins on stably transfected CHO cells (white) was analyzed by FACS upon staining with hDC-SIGN (for detection of DDDD and CCCD) or mDC-SIGN (for detection of DDDC) specific antibodies. CHO cells stably transfected with empty vector served as negative controls (black). A single experiment is shown, the results are representative for at least two independent experiments. (B) Binding of soluble EBOV-GP to chimeric lectins. Binding of a control Ig protein and of soluble EBOV-GP to CHO lines stably expressing the indicated lectins (white) or control CHO cells (black) was analyzed. A representative experiment is shown, similar results were obtained in an independent experiment.