Fig. 3.

Interactions of the wt VSV G or the VSV-G/MHV-S chimeric proteins G-STC and G-SC with the membrane protein M and their incorporation into VLPs. (A) Demonstration of intracellular interaction of the (chimeric) VSV G with M. Intracellular expression of the MHV M and E proteins in combination with the VSV G, G-STC, and G-SC protein. Radiolabeled proteins were immunoprecipitated from the cell lysate using the anti-MHV serum (α-MHV) or the anti-S monoclonal antibody (α-S) and analyzed by SDS–PAGE. (B) Demonstration of (chimeric) S protein incorporation into VLPs. Culture media were collected, processed for affinity isolation of radiolabeled VLPs using the anti-MHV serum (α-MHV) or the anti-S monoclonal antibody (α-S), and the samples were analyzed by SDS–PAGE. The molecular mass markers are indicated on the left. Arrows on the right indicate the positions of the expressed proteins.